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Cell Biology
Inhibitors
Protease Inhibitor Cocktail (100X)
Protease Inhibitor Cocktail (100X) from Cell Signaling Technology
Be the first to write a review!
Citations:
(146)
Supplier Page
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Cell Signaling Technology for
Protease Inhibitor Cocktail (100X)
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Citations
Reviews
Product Specs
Item
Protease Inhibitor Cocktail (100X)
Company
Cell Signaling Technology
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
5871S
Quantity
1 ml
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Cell Signaling Technology
3 Trask Lane
Danvers
,
Massachusetts
01923
United States
Phone
: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488
Company Profile
Website:
http://www.cellsignal.com/
Website:
Technical Support
Citations (146)
(61)
Genotoxicity and oxidative stress induction by polystyrene nanoparticles in the colorectal cancer cell line HCT116
PLOS ONE
July 23, 2021
Giulia Vecchiotti, Sabrina Colafarina, Massimo Aloisi, Osvaldo Zarivi, Piero Di Carlo, Anna Poma
buffer (cat.
9806
;
Cell Signaling
Techonology) supplemented with
protease inhibitor cocktail 100X
(cat.
5871
;
Cell Signaling
Techonology) and 1mM
PMSF
(CAT.
8553
;
Cell Signaling
Techonology). After
centrifugation
(62)
The Effects of Erchen Decoction on Gut Microbiota and Lipid Metabolism Disorders in Zucker Diabetic Fatty Rats
Frontiers in Pharmacology
July 22, 2021
Tian Zhao, Libin Zhan, Wen Zhou, Wanxin Chen, Jintong Luo, Lijing Zhang, Zebin Weng, Chunyan Zhao, Shenlin Liu
(P0012B, Beyotime, Beijing, China) supplemented with a mixture of 100 × protease
inhibitor
cocktail (
5871s
,
CST
, United States) and 100 × phosphatase
inhibitor
cocktail (
5870s
,
CST
, United States) to obtain
(63)
Tumor-derived NKG2D ligand sMIC reprograms NK cells to an inflammatory phenotype through CBM signalosome activation
Communications biology
July 22, 2021
Dhar P, Basher F, Ji Z, Huang L, Qin S, Wainwright DA, Robinson J, Hagler S, Zhou J, MacKay S,
et al
.
prepared using RIPA buffer supplemented with protease
inhibitor
cocktail (
Cell Signaling Technology
,
5871
) and phosphatase inhibitor 1 mM sodium orthovanadate. Total protein quantification of cell lysates was
(64)
eVIP2: Expression-based variant impact phenotyping to predict the function of gene variants
PLoS computational biology
July 1, 2021
Thornton AM, Fang L, Lo A, McSharry M, Haan D, O'Brien C, Berger AH, Giannakis M, Brooks AN.
(
Sigma Aldrich
, Cat. No. #
R0278
) supplemented by Protease Inhibitor Cocktail (
Cell Signaling
, Cat. No. #
5871
), then resolved by 10% SDS-PAGE.
Western blot analysis
was performed by the standard method. The
protein expression
(65)
Propionate induces intestinal oxidative stress via Sod2 propionylation in zebrafish
iScience
June 25, 2021
Ding Q, Zhang Z, Li Y, Liu H, Hao Q, Yang Y, Ringø E, Olsen RE, Clarke JL, Ran C,
et al
.
proteins" ,, 4-Hydroxy-TEMPO,Sigma,CAT#176141 Protease inhibitor cocktail,
Cell Signaling Technology
,CAT#
5871
AlarmaBlue cell viability reagent,
Invitrogen
,CAT#
DAL1025
Sodium oleate,Sigma,CAT#O7501
Palmitic acid
,
Sigma
,CAT#P5585
Sodium propionate
(66)
PRRT2 modulates presynaptic Ca
2+
influx by interacting with P/Q-type channels
Cell reports
June 15, 2021
Ferrante D, Sterlini B, Prestigio C, Marte A, Corradi A, Onofri F, Tortarolo G, Vicidomini G, Petretto A, Muià J,
et al
.
commercial
assays
,Critical commercial
assays
,, Protease inhibitor cocktail,
Cell Signaling
,Cat # #
5871
Protein G Sepharose - GE Healthcare,
Cytiva
,Cat #
17061801
EDTA-free
protease inhibitors
(Complete),Roche,Cat
(67)
LDL‑induced NLRC3 inflammasome activation in cardiac fibroblasts contributes to cardiomyocytic dysfunction
Molecular Medicine Reports
May 25, 2021
Peng Wang, Wenbo Zhang, Zhen Feng, Jian Zhang, Ying Sun, Wei Zhang
in NP-40 splitting buffer (GeneTex, Inc.; pH, 7.4) containing protease-
inhibitor
cocktail (cat. no.
5871
;
Cell Signaling Technology
, Inc.). Protein concentrations were determined using a NanoDrop spectrophotometer
(68)
SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury
iScience
May 21, 2021
Getachew A, Abbas N, You K, Yang Z, Hussain M, Huang X, Cheng Z, Tan S, Tao J, Yu X,
et al
.
,
Cell Signaling Technology
,Cat#
9806
Protease Inhibitor Cocktail (100 X),
Cell Signaling Technology
,Cat#
5871
Phosphatase Inhibitor Cocktail (100 X),
Cell Signaling Technology
,Cat#
5870
Isopropanol,"DAMAO Co.,
(69)
Continuous Monitoring of Tau-Induced Neurotoxicity in Patient-Derived iPSC-Neurons
The Journal of Neuroscience
May 12, 2021
Derek H. Oakley, Naomi Klickstein, Caitlin Commins, Mirra Chung, Simon Dujardin, Rachel E. Bennett, Bradley T. Hyman, Matthew P. Frosch
in 5× weight by volume ice-cold PBS−/− (Thermo Fisher Scientific) with protease
inhibitor
(catalog #
5871S
,
Cell Signaling Technology
) followed by
centrifugation
at 3000 × g for 10 min. The resulting pellets
(70)
EBV miRNAs are potent effectors of tumor cell transcriptome remodeling in promoting immune escape
PLOS Pathogens
May 6, 2021
Nathan Ungerleider, Whitney Bullard, Mehmet Kara, Xia Wang, Claire Roberts, Rolf Renne, Scott Tibbetts, Erik K. Flemington
Scientifc, catalog no.
10777–019
) and 10 uL of Protease Inhibitor Cocktail (
Cell Signaling
, catalog no.
5871
). Lysates were spun at 20,000g for 15 minutes at 4C. Supernatants were transferred to a new tube. For
(71)
Loss of LUC7L2 and U1 snRNP subunits shifts energy metabolism from glycolysis to OXPHOS
Molecular Cell
May 1, 2021
Alexis A. Jourdain et al.
75351
Penicillin-Streptomycin
,
ThermoFisher Scientific
,Cat #
15140122
Protease Inhibitor Cocktail (100X
),
Cell Signaling
,Cat #
5871
"
Proteinase K, Molecular Biology Grade
",
New England BioLabs
,Cat # P8107
Puromycin Dihydrochloride
,
ThermoFisher Scientific
(72)
Expression and prognostic value of E2F3 transcription factor in non-small cell lung cancer
Oncology letters
May 1, 2021
Wu L, Wan S, Li J, Xu Y, Lou X, Sun M, Wang S.
Western blot analysis
The total protein lysate was extracted from fresh tissue or cell lines using RIPA buffer (cat. no.
5871
,
Cell Signaling Technology
, Inc.). The total protein concentration was measured using a BCA protein
(73)
Myostatin Inhibition-Induced Increase in Muscle Mass and Strength Was Amplified by Resistance Exercise Training, and Dietary Essential Amino Acids Improved Muscle Quality in Mice
Nutrients
April 29, 2021
Jiwoong Jang et al.
buffer (#
9803S
,
Cell signaling Technology
, Beverly, MA, USA) containing a protease
inhibitor
cocktail (
5871S
,
Cell signaling Technology
, MA, USA) and phosphatase
inhibitor
cocktail (P5726S, Sigma-Aldrich, St Louis,
(74)
Proteomics of protein trafficking by in vivo tissue-specific labeling
Nature communications
April 22, 2021
Droujinine IA, Meyer AS, Wang D, Udeshi ND, Hu Y, Rocco D, McMahon JA, Yang R, Guo J, Mu L,
et al
.
40 μL lysis buffer (M-PER (
ThermoFisher
,
78501
) with 1× protease
inhibitor
cocktail (
CellSignaling
,
5871S
)). Cells were lysed at room temperature for 10 min and tapped to shake at the 5 min mark.
Teratomas
(75)
Adenosine A3 receptor activated in H
2
O
2
oxidative stress of primary open-angle glaucoma
Annals of translational medicine
April 1, 2021
Zhou Z, Gao Z, Yan W, Zhang Y, Huang J, Xiong K.
Technology (
CST
), Cat.
9803
] that included a protease
inhibitor
cocktail (
CST
, Danvers, MA, USA; Cat.
5871
) and PhosSTOP phosphatase
inhibitor
cocktail [MedChemExpress (MCE), Monmouth Junction, NJ, USA; Cat.4906845001].
(76)
Rimklb mutation causes male infertility in mice
Scientific reports
February 25, 2021
Maekura K, Tsukamoto S, Hamada-Kanazawa M, Takano M.
mL
cell-lysis
buffer (#
9803
,
Cell Signaling Technology
) containing a proteinase
inhibitor
cocktail (#
5871
,
Cell Signaling Technology
). The lysates were centrifuged at 12,000 rpm for 20 min at 4 °C, and the
(77)
One-shot analysis of translated mammalian lncRNAs with AHARIBO
eLife
February 17, 2021
Luca Minati et al.
RNase Inhibitor [
Thermo Scientific
#
EO0381
], 1× Protease Inhibitor Cocktail [
Cell Signaling Technology
#
5871S
]) was added to each well, and cells were lysed with the
aid
of a scraper. After hypotonic lysis, nuclei
(78)
Kras mutation rate precisely orchestrates ductal derived pancreatic intraepithelial neoplasia and pancreatic cancer
Laboratory Investigation
February 1, 2021
Kanchan Singh, Melissa Pruski, Rachael Bland, Mamoun Younes, Sushovan Guha, Nirav Thosani, Anirban Maitra, Brooks D. Cash, Florencia McAllister, Craig D. Logsdon,
et al.
cell lysis
buffer (Cell signaling #
9803
) with protease
inhibitor
cocktail tablets (
Cell signaling
#
5871
). Tissue extracts were prepared using
cell lysis
buffer (Cell signaling #
9803
) with protease
inhibitor
(79)
Crosstalk between phosphorylation and ubiquitination is involved in high salt-induced WNK4 expression
Experimental and therapeutic medicine
February 1, 2021
Zhao X, Lai G, Tu J, Liu S, Zhao Y.
lysis buffer (cat. no. P0013B; Beyotime Biotechnology, Inc.) containing
protease inhibitors
(cat. no.
5871S
;
Cell Signaling Technology
, Inc.). The cell proteins were prepared in lysis buffer containing protease
(80)
Identification of PIM1 substrates reveals a role for NDRG1 phosphorylation in prostate cancer cellular migration and invasion
Communications biology
January 4, 2021
Ledet RJ, Ruff SE, Wang Y, Nayak S, Schneider JA, Ueberheide B, Logan SK, Garabedian MJ.
AMP (
A1752
,
Sigma
), 10 mM
calyculin A
(#990;
Cell Signaling
), and 1× protease
inhibitor
cocktail (#
5871
,
Cell Signaling
) for 20 min with gentle rotation at room temperature. 150 μL of a modified 2×
radioimmunoprecipitation
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Protease Inhibitor Cocktail (100X) from Cell Signaling Technology
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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